Applications
WB
Swiss-Prot
Q9H2X6
French translation
anticorps
BiowMW
~ 110 kDa
Reactivity
Human,Mouse,Rat
Application_all
WB: 1:500~1:1000_x000D_
Group
Polyclonals and antibodies
Alternative Name
Homeodomain-interacting protein kinase 2; hHIPk2
Note
For research use only, not for use in diagnostic procedure.
Immunogen
A synthetic peptide corresponding to residues in Human HIPK2.
Specificity
HIPK2 polyclonal antibody detects endogenous levels of HIPK2 protein.
Product
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Storage&Stability
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Properties
If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Usage
Western blot (WB) analysis of HIPK2 polyclonal antibody at 1:500 dilution Lane1:Hela cell lysate Lane2:sp2/0 cell lysate Lane3:PC12 cell lysate
Purification&Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
